简介
Optosplit II 图像分离器是一种简单紧凑的设备,用于将图像分为两个独立的部分,并且可以在单个相机芯片上并排显示。
通常基于波长进行图像分离,应用于钙成像或FRET领域。两个图像可以同时捕获,相比手动或电子滤光片更换器具有更大的优势。 使用矩形孔径来定义要成像的区域,具有一组简单的控制器件,用户可以改变相机上的两个输出图像的相对位置。 Optosplit II可以显着扩大任何荧光成像系统的范围。
应用
- 离子或电压成像
- Förster共振能量转移(FRET)
- 双探针宽视野显微镜
- TIRF /旋转盘共焦
- 荧光/透射光显微镜
主要优点
- 适用于对角线高达22mm的传感器尺寸(例如5.5MPixel sCMOS)
- 用户可配置滤光片立方体
- 可选放大或缩小
- 图像不分离模式
- 独立的XY和聚焦控制
- 可容纳ND滤镜或彩色校正镜
- 标准光谱范围为450至900nm
- 支持裁剪传感器模式
相关文章
- IKCa channels control breast cancer metabolism including AMPK-driven autophagy
- Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy
- Water cycles in a Hadean CO2 atmosphere drive the evolution of long DNA
- Escherichia coli chemotaxis is information limited
- Single-molecule imaging reveals replication fork coupled formation of G-quadruplex structures hinders local replication stress signaling
- Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy
- An integrated multi-wavelength SCATTIRSTORM microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes
- E. coli chemotaxis is information-limited
- Quantitative fluorescence microscopy techniques to study three-dimensional organisation of T-cell signalling molecules
- A Multimodal Platform for Simultaneous T-cell Imaging, Defined Activation, and Mechanobiological Characterization
- Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F
- Opposing modulation of Cx26 gap junctions and hemichannels by CO2
- Endoplasmic Reticulum Lumenal Indicators in Drosophila Reveal Effects of HSP-Related Mutations on Endoplasmic Reticulum Calcium Dynamics
- Optically activated, customizable, excitable cells
- Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568