简介
OptoLED是一款稳定、高亮度的模块化LED 光源,用于荧光和透射光学显微镜,宏观和光遗传学。LED 波长范围从紫外340nm到可见光627nm。具有快速切换波长、长寿命、高稳定性、可变强度和低功耗等特点,使其成为荧光和透射光学显微镜的理想 LED光源选择。OptoLED光源具有兼容性,通过灵活的联轴器,可以与弧光灯和激光照明兼容。
OptoLED光源能够在宽范围的电流下独立驱动两个LED。对于脉冲照明,可以实现小于100ns的切换时间,并提供支持该操作模式的数字控制输入。通过前面板控制或外部控制电压设置的标准工作电流范围为0-5A。
此外,提供数字输入以独立地切换每个LED,上升和下降时间小于100ns。提供附加插座以与相机同步以减少光毒性或运动伪影。每个通道都有一个显示应用电流的仪表。
OptoLED光源还带有光学反馈功能,因为尽管LED的光输出趋向于比其他光源更稳定,但其对于温度依赖性。 在超过几毫秒的占空比的脉冲操作期间,光学反馈防止LED温度和有效波长引起输出光的变化。
OptoLED光源的具备闭环反馈控制。超高稳定性和“瞬时”(亚微秒)无振动波长切换和光强强度调节在多种应用中具有重要价值,例如组合成像和电生理学、电压敏感染料研究和高速跟踪。直接选择OptoLED光源并仅在相机采集信号时曝光样品的设施有助于减少光漂白/光损坏,并防止在读取或滚动快门伪影期间发生渗色。紫外线和白光 LED 也可用于解笼应用和明场照明。快速调制和准确定义不同波长的独立照明区域的能力使 OptoLED 光源成为光遗传学的理想光源。
应用
- 多波长荧光显微镜
- 可见/红外透射光学显微镜
- 光致遗传学
- 宏观荧光成像/光学映射
- 闪光光解
- 高速Fura-2钙成像
- 新型FuraLED耦合器
主要优点
- 高强度强度和波长稳定,光学反馈功能
- 瞬时无振动切换
- 寿命长可变强度 – 无需ND滤镜
- 低红外(热)输出
- 模块化设计满足扩展升级需求
规格
- 样品上的典型光功率可根据要求提供
- 荧光LED波长340nm,365nm,385nm,405nm,440nm **,455nm,470nm,490nm,505nm,525nm,550nm **,565nm,590nm,617nm,627nm和白色
- 可用于尼康,奥林巴斯,徕卡和蔡司显微镜的单、双、三联和四联轴器
- 通过BNC连接,TTL数字切换和模拟电压控制强度
- 输出电流1A,2A或5A,可由内部跳线链路选择
- 光反馈电路响应时间<50μsec
- 光开关时间<100nsec
- 更改外部模拟输入的响应时间<10μsec
- 外部模拟控制电压范围0到+ 10V
- **大功率白光LED
其他双通道LED光源:FuraLED / TwinLED
三款LED光源性能对比:OptoLED / MacroLED / MonoLED
近期发表的文章
- Effects of discontinuous blue light stimulation on the electrophysiological properties of neurons lacking opsin expression in vitro: implications for optogenetic experiments
- A program of successive gene expression in mouse one-cell embryos
- Region-selective control of the thalamic reticular nucleus via cortical layer 5 pyramidal cells
- A hydroalcoholic extract of Senecio nutans SCh. Bip (Asteraceae); its effects on cardiac function and chemical characterization
- In vitro longitudinal lumbar spinal cord preparations to study sensory and recurrent motor microcircuits of juvenile mice
- Electrophysiological Validation of Monosynaptic Connectivity between Premotor Interneurons and the aCC Motoneuron in the Drosophila Larval CNS
- Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells
- CaMKII inhibition reduces arrhythmogenic Ca2+ events in subendocardial cryoinjured rat living myocardial slices
- Functional characterization of ovine dorsal root ganglion neurons reveals peripheral sensitization after osteochondral defect
- Cal-520FF is the Present Optimal Ca2+ Indicator for Ultrafast Ca2+ Imaging and Optical Measurement of Ca2+ Currents
- TRPV1 feed-forward sensitisation depends on COX2 upregulation in primary sensory neurons
- Functional expression of the transient receptor potential ankyrin type 1 channel in pancreatic adenocarcinoma cells
- Differentially poised vesicles underlie fast and slow components of release at single synapses
- Age-Dependent Maturation of iPSC-CMs Leads to the Enhanced Compartmentation of β2AR-cAMP Signalling
- The Origin of Physiological Local mGluR1 Supralinear Ca2+ Signals in Cerebellar Purkinje Neurons
- An unbiased and efficient assessment of excitability of sensory neurons for analgesic drug discovery
- Angiotensin peptide synthesis and cyclic nucleotide modulation in sympathetic stellate ganglia
- Quantitative Intracellular pH Determinations in Single Live Mammalian Spermatozoa Using the Ratiometric Dye SNARF-5F
- Membrane conductances of mouse cone photoreceptors